England have named 25 players who could be selected for the game against Australia on Saturday, with Ellis Genge not included in the group.
Eddie Jones' side beat Argentina 21-8 in the first of three fixtures in November at Twickenham Stadium last weekend.
Ahead of the return of the Premiership this weekend, Genge, Tom Dunn, Piers Francis, Nick Isiekwe, George Kruis, Alex Lozowski, Zach Mercer, Marcus Smith and Denny Solomona have all returned to their clubs.
England are looking for a fifth straight win against Australia since their last defeat in October 2015.
Backs: Mike Brown, Elliot Daly, Jonny May, Semesa Rokoduguni, Anthony Watson, Danny Care, Owen Farrell, George Ford, Jonathan Joseph, Henry Slade, Ben Youngs
Forwards: Charlie Ewels, Nathan Hughes, Maro Itoje, Joe Launchbury, Courtney Lawes, Chris Robshaw, Sam Simmonds, Sam Underhill, Dan Cole, Jamie George, Dylan Hartley, Joe Marler, Mako Vunipola, Harry Williams